Comparison various level ascorbic acid and lycopene additions in semen diluent enhanced sperm quality of Sapudi ram

The primary cause of sperm quality decline during the freeze-thaw pathway is peroxidation hazard caused by reactive oxygen species produced biological molecules sperm. Ascorbic acid (Vitamin C) and lycopene are two potent antioxidants that operate to prevent oxidation processes. This study aimed analyse effects ascorbic on motility, viability, abnormality plasma membrane integrity post-thawed Sapudi rams. Sperm samples were obtained pooled from six sexually mature rams, separated into ten equal proportions diluted with TEY extender. Semen was supplemented 0 (C0; L0), 1 (C1; L1), 2 (C2; L2), 3 (C3; L3) 4 (C4; L4) mg/100mL (1–4%) diluent each lycopene, respectively. Total abnormalities semen (%) analysed after thawing. C3 L3 extenders resulted in higher total motility (<italic>p</italic><0.05) compared other extenders, all treatments than control. extender exhibited highest quality. Finally, current findings show can increase ram spermatozoa.